 |
 |
Hi Sebastian, you're thinking about local sparse matrix screening. I've done this at a 90:10 ratio. Majeed, S., Ofek, G., Belachew, A., Huang, C.C., Zhou, T., and Kwong, P.D. Enhancing protein crystallization through precipitant synergy. Structure. 2003; 11: 1061–1070 All best. Andreas On Mon, May 8, 2017 at 3:18 PM, Sebastiano Pasqualato < [log in to unmask]> wrote: > > Dear all, > I recall a paper (or was is a talk at a conference?) describing the > optimisation of initial hits of crystallisation by using a standard screen > kit as additive. > Something like setting the tray using the initial crystallisation hit > condition in the reservoir and mixing 75% of the hit condition with 25% of > a commercial sparse matrix screen kit with the protein in the drop. > I can’t find the reference, can anybody help me? > Thanks a lot, > ciao, > Sebastiano > > > -- > *Sebastiano Pasqualato, PhD* > Crystallography Unit > Department of Experimental Oncology > European Institute of Oncology > IFOM-IEO Campus > via Adamello, 16 > 20139 - Milano > Italy > > tel +39 02 9437 5167 <+39%2002%209437%205167> > fax +39 02 9437 5990 <+39%2002%209437%205990> > web http://is.gd/IEOXtalUnit > >