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Dear all, I recall a paper (or was is a talk at a conference?) describing the optimisation of initial hits of crystallisation by using a standard screen kit as additive. Something like setting the tray using the initial crystallisation hit condition in the reservoir and mixing 75% of the hit condition with 25% of a commercial sparse matrix screen kit with the protein in the drop. I can’t find the reference, can anybody help me? Thanks a lot, ciao, Sebastiano -- Sebastiano Pasqualato, PhD Crystallography Unit Department of Experimental Oncology European Institute of Oncology IFOM-IEO Campus via Adamello, 16 20139 - Milano Italy tel +39 02 9437 5167 fax +39 02 9437 5990 web http://is.gd/IEOXtalUnit