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Regarding that paper, I would point out that cytosols generally contain 50-100 mM glutamate, so it makes sense that glutamate enhances solubility.
JPK
From: CCP4 bulletin board [mailto:[log in to unmask]]
On Behalf Of Evans, Nicola
Sent: Monday, May 08, 2017 8:54 AM
To: [log in to unmask]
Subject: Re: [ccp4bb] BSA as additive
I haven't used BSA, but I did recently use 50mM L-glutamic acid for this exact reason (and 5% glycerol in all buffers except the last one for crystallography) after reading this paper and it made
a big difference to my last protein prep: https://www.ncbi.nlm.nih.gov/pubmed/15264823
For my final crystallography buffer I have tried with and without L-glutamic acid (as I am trying to optimise micro-crystals and worried the L-glu would make sample too soluble) but still waiting
to see if I get any improvement. Both have drops with micro-crystals already (after 2 days), the L-glutamic acid sample has fewer, hoping some other drops will yield better crystals over time.
Hope that helps!
Nicola
From: CCP4 bulletin board <[log in to unmask]>
on behalf of Ha Sin <[log in to unmask]>
Sent: 08 May 2017 12:32:44
To: [log in to unmask]
Subject: [ccp4bb] BSA as additive
Dear CCP4BB members,
Sorry about my non crystallography question. Does anyone know of a reference where BSA has been used as an "additive" in the protein concentration step to prevent aggregation of the main protein?
I would appreciate your suggestions.
Thank you,
H.Sin