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This looks suspiciously like Cartoon moose-head. Is it possible that you have contamination in your buffers? We once had this problem when using Canadian water. [Related image]<https://www.google.com/imgres?imgurl=http%3A%2F%2Fworldartsme.com%2Fimages%2Fmoose-face-clipart-1.jpg&imgrefurl=http%3A%2F%2Fworldartsme.com%2Fgolden-anniversary-borders-clipart.html&docid=uEQWdFqPx_AyTM&tbnid=39d_O1it6NYRlM%3A&vet=1&w=450&h=397&bih=865&biw=1580&ved=0ahUKEwim4vW834jUAhVK4IMKHb8jD2IQxiAIFigB&iact=c&ictx=1> Steve From: CCP4 bulletin board [mailto:[log in to unmask]] On Behalf Of Eleanor Dodson Sent: Wednesday, May 24, 2017 10:16 AM To: [log in to unmask] Subject: [ccp4bb] mystery feature near a PLP substrate Any ideas? Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (2000 Galloping Hill Road, Kenilworth, New Jersey, USA 07033), and/or its affiliates Direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system.