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Sorry for the confusion. I just want to avoid the hassle to go back and forth between one subunit and tetramer. My understanding is that I only need to modify one subunit of this protein and changes will be automatically applied to the other subunits if the map is changed to p4 spacegroup. I have seen people do that using MAPMAN in a couple of papers and just want to know what I am doing wrong. On Fri, May 19, 2017 at 12:15 AM, Dale Tronrud <[log in to unmask]> wrote: > > I'm sorry but I'm a little confused by your question. If your map > already has four-fold symmetry why can't you simply build your model > once in one quarter of the map? What do you hope to change by > specifying that the space group is P4? > > Dale Tronrud > > On 5/18/2017 10:06 PM, Qingfeng Chen wrote: > > Hi, > > > > I have an EM map of a tetrameric protein. It was painful to work with > > this map since it is in P1 spacegroup, although 4-fold symmetry was > > already applied during map reconstruction. > > > > I noticed that people used MAPMAN to transform spacegroup, however, it > > seems not working for me. The map remained in P1 spacegroup afterwards. > > > > I used mtz file converted from .mrc and the tetrameric protein model as > > input and choose "run fft to generate simple map". I also specified > > "output map in ccp4 format to cover all atoms in pdb". In "infrequently > > used options", I input P4 in "generate map in spacegroup". Everything > > else was left as default. > > > > Any suggestions will be appreciated. > > > > Thanks! >