 |
 |
Could you please provide more information: a) what is crystal symmetry, i.e. are you sure that you indexed consistently in the case of polar symmetries? b) what is the completeness of each data set separately? c) what is the multiplicity of observations, i.e. do you have enough data to get R-merge estimated? d) HKL2000 estimates non-isomorphism levels during scaling and prints it out. You need to use "automatic corrections" option during scaling to get that number - try it even if multiplicity is low but remember to delete the rejections file before using this option. I attached test.jpg. Everything on the left (Error Scale Factor, Error Systematic, Radiation Damage, Anomalous Signal -- even if you have none, and Internal Non-Isomorphicity) will be helpful to diagnose the problem. Could you provide these numbers or a similar screenshot? Dominika On 2017-04-10 09:46 PM, 高艺娜 wrote: > Dear all, > For my crystal, I have to merge 3-4 sets of diffraction data using > HKL2000 program to meet requirements for data completeness, but the > problem is the Rmerge value was too high to go on every time. > Did anyone have met the same problem and how to solve it? or some tips > for solve this problem？ > > All comments will be appreciated! > > Best Regards, -- Dominika Borek, Ph.D. *** UT Southwestern Medical Center 5323 Harry Hines Blvd. *** Dallas, TX 75390-8816 214-645-9577 (phone) *** 214-645-6353 (fax)