Dear Elizabeth,

The High-Throughput Crystallization Screening Center and the Hauptman-Woodward Medical Research Institute has been operating for over a decade with considerable success. There are comprehensive details at http://getacrystal.org but basically your samples are screened against a large range of commercially available conditions plus some more unique ones. Video microscope images are provided over a period of six weeks or longer by request and the imaging also includes SONICC (detecting very tiny crystals or crystals in precipitate) and UV-TPEF to ensure the sample is protein. There are a large range of analysis tools that can be used on the data. Much of the information is provided on the link above. Success rates are pretty high and many entries in the PDB have had their initial crystal hits there.

I hope this helps, the center does not advertise much but works with a lot of laboratories.

Cheers,

Eddie.

Edward Snell Ph.D.

President and CEO Hauptman-Woodward Medical Research Institute

Assistant Prof. Department of Structural Biology, University at Buffalo

700 Ellicott Street, Buffalo, NY 14203-1102

hwi.buffalo.edu

Phone: (716) 898 8631 Fax: (716) 898 8660

Skype: eddie.snell Email: [log in to unmask]

Heisenberg was probably here!

From: CCP4 bulletin board <[log in to unmask]> on behalf of Elizabeth Diaz <[log in to unmask]>
Sent: Friday, April 14, 2017 9:06 AM
To: [log in to unmask]
Subject: [ccp4bb] Recommendations for Robotic Crystal Screening Services

All,

I am currently attempting to crystallize a peptide/protein complex and am wanting to know about robotic screening services that you would recommend. We have done some in house conditions with little luck, and want to broaden our search, but that would require going externally for screening. Do you have any recommendations for robotic crystal screening services, preferably in the United States?

Thank you so much,

Elizabeth Diaz

University of Delaware