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Darn it, you're right. JPK -----Original Message----- From: CCP4 bulletin board [mailto:[log in to unmask]] On Behalf Of Hughes, Jon Sent: Thursday, March 30, 2017 4:05 AM To: [log in to unmask] Subject: [ccp4bb] AW: [ccp4bb] [ccp4bb] protein precipitation reg yes, "oil of vitriol" is sulphuric acid, "blue vitriol" is copper II sulphate as i recall. j -----Ursprüngliche Nachricht----- Von: CCP4 bulletin board [mailto:[log in to unmask]] Im Auftrag von CRAIG A BINGMAN Gesendet: Donnerstag, 30. März 2017 05:52 An: [log in to unmask] Betreff: Re: [ccp4bb] [ccp4bb] protein precipitation reg Since I’m in full-on cranky old biochemist mode now, I think that vitriol is an old name for sulfuric acid. > On Mar 29, 2017, at 8:40 PM, Keller, Jacob <[log in to unmask]> wrote: > >> And if we are going to pour scorn and vitriol on Tris, why not mention its large dpKa/dT of 0.03 pH units/deg ? > > Hah! That's what many people are doing when they make buffers: pouring vitriol (HCl) on TRIS! I prefer to pour concentrated HEPES, and get two buffers without adding any extra Cl-. > > JPK