Dear all,
I am trying to obtain crystals of membrane complex. Now, I have obtained the complex with appropriate stoichiometry after a series of purification in DDM, then I get extremely thin needles (as shown in figure) in some conditions such as 28% MPD, 0.1 M NaAc pH 4.5 or 45% MPD,0.1 M Bis-Tris pH 6.5, 0.2 M CaCl2. However, there are not any improvements through changing the concentration of precipitants/salts or adding the additives/detergents to the protein.
I would be very happy if anybody could give me some advice about this crystal refinement.
With best regards.
