 |
 |
Automated pipelines for compound and fragment screening in Grenoble The EMBL Grenoble, the ESRF and the iNEXT consortium are jointly offering access to automated pipelines for compound and fragment screening based on the CrystalDirect* technology and the MASSIF** beamlines. Access to these pipelines can be obtained through the E.C. H2020 project iNEXT (www.inext-eu.org). Applications will be reviewed by the iNEXT scientific review panel in collaboration with the ESRF MX Beamtime Allocation Panel. These pipelines are offered in two forms: Compound Screening Pipeline: Automated screening via X-ray crystallography of a collection of small molecule ligands identified through previous assays (i.e in silico, screening, activity assays, SPR, etc.). Applicants must be able to provide the ligand collection, pure protein in sufficient amounts and well-established and robust crystallization conditions. Fragment Screening pipeline: Automated large scale fragment screening via X-ray crystallography. The fragment collection will normally be provided by the Grenoble facility although screening of user provided libraries is also possible. Applicants must be able to provide pure protein in sufficient amounts and well-established and robust crystallization conditions Additionally, we are also offering access to an Integrated Macromolecular Crystallography pipeline offering fully automated, remote controlled access to crystallization screening, optimization of crystal conditions, automated crystal mounting and cryocooling (based on EMBL'S CrystalDirect technology and automated (or manual) data collection at the ESRF MASSIF-1 or MASSIF-3 beamlines. To apply visit the iNEXT web page (www.inext-eu.org) or contact us. * Automated harvesting and processing of protein crystals through laser photoablation Zander, U., Hoffmann, G., ... & Marquez, J. A. (2016). Acta Cryst. D72, 454-466. https://doi.org/10.1107/S2059798316000954 ** Svensson O, Monaco S, Popov AN, Nurizzo D, Bowler MW (2015). The fully automatic characterization and data collection from crystals of biological macromolecules, Acta Cryst. D71, 1757-1767, doi:10.1107/S1399004715011918 -- _________________________________________________________ Jose A. Marquez Ph.D. Team Leader, Head of the Crystallization Facility EMBL Grenoble Outstation Postal address: European Molecular Biology Laboratory 71, Avenue des Martyrs CS 90181 38042 Grenoble Cedex 9, France Delivery address: European Molecular Biology Laboratory 71, Avenue des Martyrs 38000 Grenoble, France Phone +33 (0)476 20 74 25 Fax. +33 (0)476 20 71 99 https://embl.fr/htxlab/ _________________________________________________________