To follow up on this issue:

It seems that only when not downscaling or lowpass filtering the input files, does the manual picking work as it should. So we think there may be a problem performign scaling/lowpass filtering on the input micrographs. Interestingly, scaling worked fine with the betagal test set images. Could this be a fftw problem?

Thanks in advance!

On Mon, Feb 13, 2017 at 2:14 PM, David Boyer <[log in to unmask]> wrote:

Hello,

I have successfully completed relion 2.0 tutorial, so I believe my software should be working properly. However, when I start to process my own data, I am getting stuck at trying to manually pick some example particles. When I hit the "pick" button the the manual-picking GUI, the program takes a good while (several minutes) to display anything, and when it does, it is only a completely black "micrograph." When I hit the "CTF" button on the manual-picking GUI, it quickly displays the power spectrum and CTF model for that micrograph.

I have tested again that when I use the betagal test data and manual-picking with my current build of relion, the GUI works correctly and promptly displays the micrograph for manual particle picking.

Does anyone have suggestions as to how to overcome this roadblock?

Thanks,

David