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Hello Eleanor, We found some intermolecular vicinal disulfides recently that we think are 'real'. This class of proteins forms tetramers and in one version we find these intermolecular disulfides across molecules. We did some tests and found that oxidation or reduction has an effect on the stability of the protein. ?We also saw this was consistent across multiple space groups. If you would like to have a look, they were just released: 5HY0, 5HY2, 5HY4. As a comparison to another protein in this fold class that doesn't have the disulfide is 5HWE. cheers, tom Tom Peat Proteins Group Biomedical Program, CSIRO 343 Royal Parade Parkville, VIC, 3052 +613 9662 7304 +614 57 539 419 [log in to unmask] ________________________________ From: CCP4 bulletin board <[log in to unmask]> on behalf of Eleanor Dodson <[log in to unmask]> Sent: Thursday, February 2, 2017 2:17 AM To: [log in to unmask] Subject: [ccp4bb] intermolecular dissulphides Does anyone know of examples of these? I have found one - 2WQW with these SSBOND records 2WQW SSBOND 1 CYS A 206 CYS A 227 1555 6556 2.07 SSBOND 2 CYS B 206 CYS B 227 1555 5556 2.15 We seem to have one but it would have to form after crystalisation? Eleanor