On Feb 10, 2017, at 1:30 PM, Eleanor Dodson <[log in to unmask]> wrote:

Well - in my experience it gives much clearer density for missing features..

E

On 10 February 2017 at 10:59, Kajander, Tommi A <[log in to unmask]> wrote:

Dear Eleanor, I will try that, just wondering if excluding solvent masking completely doenst have detrimental effect on

the overall quality of the map?

Tommi

On Feb 10, 2017, at 12:43 PM, Eleanor Dodson <[log in to unmask]> wrote:

Certainly if you are using REFMAC with an incomplete model you should specify

SCALE TYPE BULK

SOLVENT NO

Otherwise it assumes any density outside the given model is to be effectively screened out..

Eleanor

On 10 February 2017 at 09:37, Kajander, Tommi A <[log in to unmask]> wrote:

Hi All, 

Was there a convenient  way to make a solvent mask for a region with a model  - its been a while - and use that to generate maps 

(i have a domain that is only partially visible, could not be found be molecular replacement), its there though. 

Could be that its not well ordered, but I was wondering if the bulk solvent masking is just wiping it out. (basicly something like half a domain, e.g. half of 

individual beta-strands, are missing.) resolution is bit limited (at best 3 Å) so automated building and refinement doesnt work terribly well.

I could just place a model there and make a mask somewhere and include in map calculation?

Thanks for suggestions,

tommi