Hi all,

 

Many thanks to Sarah Hodge, my lab has deposited 32 clock mutant and/or transgenic stocks at uNASC since last October. They are now online, many thanks to the curators and team at the Eurasian stock centre, uNASC. NASCIDs are N2107351-69 and N2107700-12 e.g. http://arabidopsis.info/StockInfo?NASC_id=2107360

 

Some of these are mutant alleles from the EMS mutant screen originally reported in Kevei et al. Plant Journal 2006. http://www.plantphysiol.org/content/140/3/933/F2.expansion.html

 

They include the rare, early-flowering point mutant of GIGANTEA gi-611, reported in Supplementary Figure 3 of Gould et al. Plant Cell 2006, along with gi-596, both of which we thought were lost. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1456873/bin/plntcell_tpc.105.039990_index.html

 

Sarah recovered some of these lines by systematically testing all of our older stocks.

Why not deposit earlier? *Cautionary tale*: I delayed depositing at NASC because I was waiting to retrieve homozygotes after more than two back-crosses. However, depositing usable stocks reliably and early is more important than more back-crossing to remove second-site mutations. One can always send in the perfect line later.

 

So come on, clock folks, take half an hour next week to send in your lines!

 

Cheers,

Andrew

 

This email duplicates tweets

https://twitter.com/A_J_Millar/status/827563850220331008

https://twitter.com/A_J_Millar/status/827562768953909248

 

 

Prof. Andrew J. Millar FRS FRSE

SynthSys

C.H. Waddington Building

University of Edinburgh

Edinburgh EH9 3BF

Scotland, UK

 

Tel +44 131 651 3325

@A_J_Millar; www.amillar.org; www.synthsys.ed.ac.uk