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Dear Chih-Hao, This is to be expected for a model that includes subject effects. The corresponding estimable contrast is [1 0 0 0 0 0 1/3 1/3 1/3 0 0 0] but you are essentially trying to do a one-sample t-test with three subjects so you should acquire data from more subjects before running a second level model. Best regards, Guillaume. On 11/01/17 06:55, 連 志浩 wrote: > Dear experts, > > > I have a problem when I tried to use flexible factorial design. > > > All the bars below the design matrix are gray, and I can't do any contrast. > > > GUI showed "no contrast(s)" when I open SPM contrast manager, and showed > "!invalid contrast" when I try to input any contrast. > > > It's a 2 x 4 flexible factorial design with a Group factor with 2 level > and a Condition factor with 4 level, and I set 3 factors in my model: > > > Factor 1: Subject, Independence: Yes, Variance: Equal > > Factor 2: Group, Independence: Yes, Variance: Unequal > > Factor 3: Condition, Independence: No, Variance: Equal > > > I would appreciate for any help. > > > Thanks for your reading. > > > Best, > > Chih-Hao > > > > > > > -- Guillaume Flandin, PhD Wellcome Trust Centre for Neuroimaging University College London 12 Queen Square London WC1N 3BG