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Hi Faezeh, I recommend to get the b0 images from both dataset (orig_b0 and new_b0). Then (I assume they were acquired from the same subject) use rigid-body registration (Coregister): - reference/target: orig_b0 - source: new_b0 - other: new_FA Vale, Tibor Auer, Tibor M.D. Ph.D. Research Fellow in MRI Department of Psychology Royal Holloway University of London Egham TW20 0EX United Kingdom +44-1784-27-6594 [log in to unmask] -----Original Message----- From: SPM (Statistical Parametric Mapping) [mailto:[log in to unmask]] On Behalf Of Faeze Vedaei Sent: Wednesday, January 18, 2017 3:30 PM To: [log in to unmask] Subject: Re: [SPM] Coregistration Issue Dear Dr. Tibor Thank you for your response. Actually, I do not know whether the way to do this coregistration (FA map to this DTI data) which I did gave the accurate result or not (you can see from the screenshots I sent in my first email). Maybe coregistration of original DTI data to the new DTI data (dwi data) could be a good idea. May you let me know which of these methods you would recommend and why? and if I want to apply registration parameters (of original DTI to dwi data) to FA map, how can I do that? I truly appreciate your time and help me in this regard. Bests, Faezeh