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Hello, I have created an unnatural amino acid (ua1.pdb, ua1.cif) in JLigand, I have added it to my structure and fitted it into the density. Now how do I tell Coot, when I regularise the local structure, to keep the peptide bond stereochemistry with the preceding and following amino acids? Thanks for the help. Best wishes, Luca Luca Pellegrini, PhD Department of Biochemistry University of Cambridge 80 Tennis Court Road Cambridge CB2 1GA UK Email: [log in to unmask] Phone: 0044-1223-760469