Hi Rado, Please try V1.06. It is more flexible for phase shift determination and contains different options to control the target function and refinement parameters. http://www.mrc-lmb.cam.ac.uk/kzhang/Gctf/ Best wishes, Kai 发件人:Radostin Danev <[log in to unmask]> 发送时间:2017-01-30 18:02 主题:Gctf with phase shift 收件人:"kzhang"<[log in to unmask]> 抄送: Hi Kai, I was wondering if you have a newer version of Gctf with phase plate phase shift support? The one on you website (v0.50) says to use amplitude contrast of 1.0 for phase plates, but this is not optimal for the Volta phase plate. Cheers, Rado =================================== Radostin Danev, Ph.D. Max Planck Institute of Biochemistry Department of Molecular Structural Biology Am Klopferspitz 18 82152 Martinsried Germany e-mail: [log in to unmask] phone: +49 (89) 8578-2651 fax: +49 (89) 8578-2641 ===================================