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Can I just make the point that the subject "Completely off-topic" is completely useless if you're searching through the archives for anything? Since it's attracted quite a bit of correspondence, this obviously isn't off-topic at all! Harry -- Dr Harry Powell Chairman of International Union of Crystallography Commission on Crystallographic Computing Chairman of European Crystallographic Association SIG9 (Crystallographic Computing) > On 12 Jan 2017, at 13:24, David Schuller <[log in to unmask]> wrote: > > I wonder if anyone has tried glutamate as a cryprotectant for proteins. If the [] is that high, it may make a stable environment for proteins. I have no idea what effect it might have on ice formation. > > https://amb-express.springeropen.com/articles/10.1186/2191-0855-3-36 > Bacillus subtilis natto: a non-toxic source of poly-γ-glutamic acid that could be used as a cryoprotectant for probiotic bacteria > > > > Bhat, et al DOI: 10.1186/2191-0855-3-36 > > > > > >> On 01/11/2017 07:45 PM, Keller, Jacob wrote: >> Dear Crystallographers, >> >> Was anyone else aware that in E coli the intracellular glutamate concentration is ~100 mM? Also other cell types (yeast, mammalian) are 10s mM. Anything to say about this? I learned of this just recently, and have been amazed about it for more than a week. Did I miss this in Biochem 101? Does it matter? >> >> JPK >> >> ******************************************* >> Jacob Pearson Keller, PhD >> Research Scientist >> HHMI Janelia Research Campus / Looger lab >> Phone: (571)209-4000 x3159 >> Email: [log in to unmask] >> ******************************************* >> > > > -- > ======================================================================= > All Things Serve the Beam > ======================================================================= > David J. Schuller > modern man in a post-modern world > MacCHESS, Cornell University > [log in to unmask]