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I wonder if anyone has tried glutamate as a cryprotectant for proteins. If the [] is that high, it may make a stable environment for proteins. I have no idea what effect it might have on ice formation.

https://amb-express.springeropen.com/articles/10.1186/2191-0855-3-36

Bacillus subtilis natto: a non-toxic source of poly-γ-glutamic acid that could be used as a cryoprotectant for probiotic bacteria



Bhat, et al DOI: 10.1186/2191-0855-3-36





On 01/11/2017 07:45 PM, Keller, Jacob wrote:
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Dear Crystallographers,

 

Was anyone else aware that in E coli the intracellular glutamate concentration is ~100 mM? Also other cell types (yeast, mammalian) are 10s mM. Anything to say about this? I learned of this just recently, and have been amazed about it for more than a week. Did I miss this in Biochem 101? Does it matter?

 

JPK

 

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Jacob Pearson Keller, PhD

Research Scientist

HHMI Janelia Research Campus / Looger lab

Phone: (571)209-4000 x3159

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