I wonder if anyone has tried glutamate as a cryprotectant for proteins.
If the [] is that high, it may make a stable environment for proteins. I
have no idea what effect it might have on ice formation.
https://amb-express.springeropen.com/articles/10.1186/2191-0855-3-36
/Bacillus subtilis/ natto: a non-toxic source of poly-γ-glutamic acid
that could be used as a cryoprotectant for probiotic bacteria
Bhat, et al *DOI: *10.1186/2191-0855-3-36
On 01/11/2017 07:45 PM, Keller, Jacob wrote:
>
> Dear Crystallographers,
>
> Was anyone else aware that in E coli the intracellular glutamate
> concentration is ~100 mM? Also other cell types (yeast, mammalian) are
> 10s mM. Anything to say about this? I learned of this just recently,
> and have been amazed about it for more than a week. Did I miss this in
> Biochem 101? Does it matter?
>
> JPK
>
> *******************************************
>
> Jacob Pearson Keller, PhD
>
> Research Scientist
>
> HHMI Janelia Research Campus / Looger lab
>
> Phone: (571)209-4000 x3159
>
> Email: [log in to unmask] <mailto:[log in to unmask]>
>
> *******************************************
>
--
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All Things Serve the Beam
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David J. Schuller
modern man in a post-modern world
MacCHESS, Cornell University
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