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On 1/19/17 3:54 PM, Panneerselvam, Saravanan wrote: > We observed additional density around ADP that fits perfectly > like a gamma phosphate Hello Saravanan At 1.4 Angstrom resolution wouldn't that suggest that you've somehow got ATP in there ? I don't think I understand the other option - were you proposing a ADP-O-C(O)2 arrangement to explain the density ? Surely that has a rather different shape, considerably different scattering power at the center of the terminal group (C vs P) and probably different X-O bond lengths. All of these should show in the density maps at 1.4 Å, although the bond length issue could be quite subtle. Phil Jeffrey Princeton > mimicking like ATP bound state, surrounded and > coordinated by two metal ions(resolution is 1.4A). There is a change in > space group (from I212121 to P212121 ) and further important > conformation changes are observed around ATP binding pocket and distant > region. This is the only xtal we obtained in this space group, and all > other xtals(measured 10 xtals) from the same plate belong to I212121. > > > > Thanks your help and time! > > Saravanan