Try Tricine gel. It is particularly suited for low molecular wt proteins and it will give you very good resolution. Even you can run it overnight at 30 V (16-18 hours).

On Thu, Jan 19, 2017 at 9:33 AM, Walt <[log in to unmask]> wrote:

Hi,

I have a small protein (~9 kDa) with acidic pI (~4).

When I run 18% native-PAGE, it appears my protein is in the dye front.

How can I fix this problem? Changing the pH of separating gel

might help? How about gradient native-PAGE? Thank you!

Walt