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Yes Tris-Tricine gel ! Try to obtain this article from nature protocol. Best, Didier 2017-01-19 14:47 GMT+01:00 zeyaul islam <[log in to unmask]>: > Try Tricine gel. It is particularly suited for low molecular wt proteins > and it will give you very good resolution. Even you can run it overnight at > 30 V (16-18 hours). > > On Thu, Jan 19, 2017 at 9:33 AM, Walt <[log in to unmask]> wrote: > >> Hi, >> >> I have a small protein (~9 kDa) with acidic pI (~4). >> When I run 18% native-PAGE, it appears my protein is in the dye front. >> How can I fix this problem? Changing the pH of separating gel >> might help? How about gradient native-PAGE? Thank you! >> >> Walt >> > > -- Didier Spittler, PhD Phone number : +33658576481