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A) change dye or use pure glycerol to load gel B) change gel pH or add various substances to it (easy to do with the buffer) Artem On Jan 19, 2017 6:34 AM, "Walt" <[log in to unmask]> wrote: > Hi, > > I have a small protein (~9 kDa) with acidic pI (~4). > When I run 18% native-PAGE, it appears my protein is in the dye front. > How can I fix this problem? Changing the pH of separating gel > might help? How about gradient native-PAGE? Thank you! > > Walt >