JISCMail - ACB-CLIN-CHEM-GEN Archives

Our lower value of 2.0 ug/L is the 5th percentile of a healthy adult (male and female) reference population who had no previous history of anaemia or abnormal haematinics.

The “calculated lower reference limit” (based on the regression equation of patient comparisons of the previous Roche and new Roche folate III that were done in our laboratory) was 1.7 ug/L.

Based on the similarity of these two values our haematologist colleagues decided that 2.0 would be a pragmatic choice.

Kind regards

Ian

From: Leifur Franzson [mailto:[log in to unmask]]
Sent: 12 January 2017 08:59
To: BARLOW, Ian (NORTHERN LINCOLNSHIRE AND GOOLE NHS FOUNDATION TRUST)
Cc: [log in to unmask]
Subject: RE: Roche - restandardised folate III method reference ranges

This is indeed very low compared to expected values from the kit insert.

Folate reference values may be difficult to determine due to seasonal intake of vegetables etc

Most favourably folate reference (lower limit) should be the concentration where deficiency symptomes i.e. anemia etc occurs.

Is your value based on general distribution ?

Best regards

Leifur Franzson
Reykjavik
Iceland

From: "BARLOW, Ian (NORTHERN LINCOLNSHIRE AND GOOLE NHS FOUNDATION TRUST)" <[log in to unmask]>
To: Leifur Franzson <[log in to unmask]>, "[log in to unmask]" <[log in to unmask]>
Date: 11.01.2017 16:32
Subject: RE: Roche - restandardised folate III method reference ranges

Our locally derived lower ref limit in Lincolnshire, UK, is 2.0ug/L

Best wishes

Ian

From: Clinical biochemistry discussion list [mailto:[log in to unmask]] On Behalf Of Leifur Franzson
Sent: 11 January 2017 15:08
To: [log in to unmask]
Subject: Re: Roche - restandardised folate III method reference ranges

Interesting discussion.

What reference values are the Roche users using for serum/plasma folate with the new reagents ?

Best regards

Leifur Franzson MScPharm
Specialist in Clinical Biochemistry
Department of Genetics and Molecular Medicine
Landspitali, Hringbraut
Iceland

From: "Dr. Sutirtha Chakraborty" <[log in to unmask]>
To: [log in to unmask]
Date: 19.12.2016 17:03
Subject: Re: Roche - restandardised folate III method reference ranges
Sent by: Clinical biochemistry discussion list <[log in to unmask]>

I share the same concerns......
Folate deficiency is very rare in India. We are the champions at eating green.
However we have faced a sudden increase in folate deficiency with the folate III assay on Roche e411.This transition to the new folate assay gen has occurred recently, so I dont have much specific data on the percentage of deficiency.

Regards,
Sutirtha

Dr Sutirtha Chakraborty, MBBS, MD, FACB
Chief Consultant, Department of Biochemistry
Peerless Hospital & B K Roy Research Center
360, Panchasayar, Kolkata 700094, WB, India

On Mon, Dec 19, 2016 at 8:52 PM, Sally Slack <[log in to unmask]> wrote:

Dear Roche users,

We have recently moved on to the re-standardised folate III method and have applied the reference interval suggested by Roche (3.9-26.8ng/mL).

As a consequence of this we are now reporting 33% of results below the folate reference interval.

Has anyone got an evidence based reference range for the re-standardised folate III method, based on a UK population?

We may be able to get around this using a transference equation, but it would be good to hear what reference intervals other Roche users have in place.

North Cumbrian folk aren’t the best at eating their greens but we can’t all be folate deficient!

Many thanks,

Sally

Sally Slack MBIOCHEM, MSc, FRCPath

Consultant Biochemist

Blood Sciences

North Cumbria University Hospitals NHS Trust,

Cumberland Infirmary,

CARLISLE, CA2 7HY.

Tel: 01228 814521 Fax: 01228 814831

The NCUHT Pathology Handbook can be found here

For general information on tests see Lab Tests Online

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------ACB discussion List Information-------- This is an open discussion list for the academic and clinical community working in clinical biochemistry. Please note, archived messages are public and can be viewed via the internet. Views expressed are those of the individual and they are responsible for all message content. ACB Web Site http://www.acb.org.uk Green Laboratories Work http://www.laboratorymedicine.nhs.uk List Archives http://www.jiscmail.ac.uk/lists/ACB-CLIN-CHEM-GEN.html List Instructions (How to leave etc.) http://www.jiscmail.ac.uk/

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http://www.landspitali.is/disclaimer/ ------ACB discussion List Information-------- This is an open discussion list for the academic and clinical community working in clinical biochemistry. Please note, archived messages are public and can be viewed via the internet. Views expressed are those of the individual and they are responsible for all message content. ACB Web Site http://www.acb.org.uk Green Laboratories Work http://www.laboratorymedicine.nhs.uk List Archives http://www.jiscmail.ac.uk/lists/ACB-CLIN-CHEM-GEN.html List Instructions (How to leave etc.) http://www.jiscmail.ac.uk/