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Dear experts, We want to investigate interaction between Groups (ex. normal and patients) and a continuous variable X. I think I should do these followed steps: 1. Use 2nd-level modeling, and choose "Two-sample t-test". 2. Input normal subjects' .con image into "Group 1 scans" and patients' .con image into "Group 2 scans", then set Variance as "Unequal". 3. Create a covairate (variable X) for all subjects. 4. Run 2nd-level modeling and estimate its' SPM.mat file. 5. Reulst -> difine a t-contrast -> vector [ 0 0 1] then I can get the interaction between Group and Variable X. Is anything wrong is my steps? or there's some better ways to analysis the interaction I want to investigate? Thanks for your reading. Best, Chih-Hao