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Hi All, I have an ANOVA analysis problem using randomise. I have 21 subjects data and each subject was measured twiced. These 21 subjects are divided into three groups, i.e group1=8 subjects, group2=6 subjects, group3=7 subjects. Would anyone know how to design the matrix? My input data is a 4D matrix, i.e. volume_3d*42, where the first 21 volume_3d are measured at the first time and the order is accoring to the group1-3. The second 21 volume_3d are measured at the second time and the order is the same. Thanks very much. Zheng