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Hi Veronica,

your data seem OK. Though, your high res cc is unusually low  taken all the other values into account. Is that a copy paste error? Anyway your two other domains look pretty decent, which indicate that your solution is correct. The B-factors for your good bits are already quite high (around 90 if I identify that correct from the screenshots). Maybe your third domain is very disordered and can't be built as good as you hope and you might have to live with just a few bits of it, which might be better ordered.

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Dear all, Thanks for your valuable comments and ideas.

1. Data were collected to 2.5 A (Peak, Inflection, Remote)

2. Solved with Sharp

3. Protein (we think has 1-2-3 rigid domains). Bucaneer built the 2,3 domains (small domains).

4. I made a Chainsaw model from sequence-structure search in NCBI-BLAST for domain 1.

The 2, 3 domains seem to be OK without breaks. I have attached the coot screenshots. I worry if the phases are totally wrong? is it possible that phases are wrong but I get 2 correct domains.

Any advice for me...

Aimless log file:
Low resolution limit                       62.77     62.77      2.49
High resolution limit                       2.40      8.98      2.40

Rmerge  (within I+/I-)                     0.082     0.034     2.000
Rmerge  (all I+ and I-)                    0.106     0.063     2.097
Rmeas (within I+/I-)                       0.089     0.038     2.200
Rmeas (all I+ & I-)                        0.111     0.066     2.195
Rpim (within I+/I-)                        0.035     0.015     0.900
Rpim (all I+ & I-)                         0.031     0.019     0.641
Rmerge in top intensity bin                0.035        -         -
Total number of observations              481476      9467     45296
Total number unique                        38944       774      4032
Mean((I)/sd(I))                             14.2      60.9       1.3
Mn(I) half-set correlation CC(1/2)         0.999     0.999     0.087
Completeness                                99.8      99.9      98.9
Multiplicity                                12.4      12.2      11.2

Anomalous completeness                      99.3      99.6      96.8
Anomalous multiplicity                       6.1       6.3       5.6
DelAnom correlation between half-sets      0.865     0.893    -0.023
Mid-Slope of Anom Normal Probability       1.273       -         - 


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