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Hi, Thank you for the comment, I will just concentrate on improving the resolution. Best regards, Kotaro Tanaka > -----Original Message----- > From: Sjors Scheres [mailto:[log in to unmask]] > Sent: Thursday, November 03, 2016 5:05 PM > To: Kotaro Tanaka <[log in to unmask]> > Subject: Re: [ccpem] Relion2: Artefact by helical symmetrizing operation? > > Hi Kotaro, > These should go away with the next iterations when the model gains > resolution. > HTH, > Sjors > > > Dear all, > > > > I'm performing helical reconstruction with relion2. > > > > I noticed that in the reconstructed volume, the strange lines > > perpendicular to the helical axis show up. I hope you can see them in > > the attached image. > > The neighboring lines are separated by ~helical rise, so I suspect > > that it is an artefact by helical symmetrizing operation. > > > > The helical symmetry of the specimen is already well established, so > > the problem does not seem to be caused by specifying wrong helical > parameters. > > > > I will appreciate if you give me any comment about this. > > > > Best regards, > > Kotaro Tanaka > > > > > > > -- > Sjors Scheres > MRC Laboratory of Molecular Biology > Francis Crick Avenue, Cambridge Biomedical Campus Cambridge CB2 0QH, > U.K. > tel: +44 (0)1223 267061 > http://www2.mrc-lmb.cam.ac.uk/groups/scheres