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Hi,

That message should mean that you didn't ask for a number of molecules divisible by the order of the tNCS. With that vector you need to search for an even number of copies.

Let me know if that doesn't explain what you're saying. 

Best wishes,
Randy Read 

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Randy J. Read

On 25 Nov 2016, at 21:27, KAUSHIK H.S. <[log in to unmask]> wrote:

Hello,

I have a crystal in C2 spacegroup (94.2073 148.003 72.9967 90 97.6686 90) and Xtriage predicts 923 residues in ASU.  My protein could be anywhere between 95 to 110 residues long (assuming the terminals could be cleaved).  Using a homolog, Phaser gives me a solution with LLG=1072 and TFZ=45.  Molrep placed 4 protomers in the ASU however, the crystal packing is poor.

I understand from Phaser that the tNCS is not accounted for (because the structure is linear?).  Is there a way the exact location of the tNCS related protomers be predicted using information from SfCheck and Xtriage? 

Xtriage information:
Fraqc. coord. : 0.500 0.000 0.000
Distance to origin: 47.104
Height relative to origin: 79.688%
p_value(height) : 5.283e-07

SFCHECK:
Pseudo-translation is detected: 62.6%
Pseudo-translation vector: 0.500 0.000 0.000

Sorry if my question is too naive.

Thanks in advance,
Kaushik Hatti,
Molecular Biophysics Unit,
Indian Institute of Science,
India.