Dear everyone:
Recently, I suffered a problem during my research work. I purified a zinc finger protein, and crystallized as a beautiful cube in a reservoir solution only containing phosphate as the precipitant, no other buffer or molecules. However, regardless of multiple optimization, the crystal diffracted badly (7~8 Å best). I have also tried co-crystallization with dsRNA because this protein can bind to dsRNA. Then crystals grow in a new condition(2.5M (NH4)2SO4,0.1M BTP, pH7.0)and its form change to cluster of needle. But the X-ray diffraction diagram is very strange(as shown in the picture). The Data cannot be processed with HKL2000 either. I want to figure out, could this be a RNA crystal rather than the complex? Or is there anybody know about the crystal of RNA molecular?
Thank you very much!
Yujie Liu
Room 2071, research center in life sciences,
China Agricultural University
No. 2 yuanmingyuan west road, Haidian District, Beijing, 100193 P.R. China
Tel: (86)-10-62734078