Hi Alex,

You may also think of gradually increasing PEG3350 concentration to around 40% which has worked pretty well in some of my cases.

Greetings,
Abhi

Abhimanyu Kumar Singh, Ph.D.
School of Biosciences
University of Kent
Canterbury, UK.

On 27 Sep 2016 23:43, "Alex Lee" <[log in to unmask]> wrote:

Hi CCP4bb members,

I have a condition for my protein crystals at "0.2M sodium thiocyanate, 25% PEG3350, pH6.9".  

I'd like to try cryoprotectant with just higher concentration of sodium thiocyanate, but I have no google hits of the range of concentration I should use. 

I wonder anyone in this forum tried to use sodium thiocyanate as cryoprotectant?  What's the concentration range for this if used as cryoprotectant?

Thanks.