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Probably you can not use thiocyanate as cryoprotectant because its a chaotropic agent. Although its a very mild denaturant (destabilizer) but at high concentration, it can destroy your crystals. I have recently used 30 % sucrose for cryo protection of crystals growing under same condition and it worked beautifully. My crystals were cracking in glycerol. 

Dhiraj 

On Sep 27, 2016, at 5:43 PM, Alex Lee <[log in to unmask]> wrote:

Hi CCP4bb members,

I have a condition for my protein crystals at "0.2M sodium thiocyanate, 25% PEG3350, pH6.9".  

I'd like to try cryoprotectant with just higher concentration of sodium thiocyanate, but I have no google hits of the range of concentration I should use. 

I wonder anyone in this forum tried to use sodium thiocyanate as cryoprotectant?  What's the concentration range for this if used as cryoprotectant?

Thanks.