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Even with no anomalous signal by conventional measures, Phaser can find anomalous atoms using partial model phases, since the phases really boost the signal. On the other hand, if there's not a single anomalous atom in the structure, well... I would just try it and see what happens. JPK -----Original Message----- From: CCP4 bulletin board [mailto:[log in to unmask]] On Behalf Of Abhishek Jalan Sent: Tuesday, September 20, 2016 2:09 AM To: [log in to unmask] Subject: Re: [ccp4bb] multiple similar solutions in phaser Hi Both xtriage and pointless suggest a C2 space group. When I tried phaser with the same data indexed in C2 space group, it yields two solutions (LLG=672, TFZ=7.6) both containing a single triple helix in the asymmetric unit. This corresponds to a rather low solvent content of 68% for such a resolution. Refinement statistics for the top solution in C2 are not encouraging either. start final --------------------------------------- R-work: 0.5522 0.5020 R-free: 0.5162 0.5403 RMS(angles): 1.82 1.80 RMS(bonds): 0.013 0.011 Where can I get a polyA model to try in phaser. Can I build one in pymol and use it? Unfortunately, there is no anomalous signal.