Dear community,
the autonomous data collection beamline MASSIF-1 at the ESRF has
now been running for 2 years and is highly successful. The service
is available for all types of sample mount and crystals, from
screening initial hits from crystallisation screens through to the
collection of hundreds of data sets from well characterised
systems. Essential points:
1. Time is not scheduled but booked on-line when you need it via
an online calendar in consultation with your BAG responsible
- you can book regular slots or at short notice when you get
crystals
2. If you are not part of a BAG you are welcome to apply for rapid
access time here http://www.esrf.eu/MXnon-BAGproposal
3. No restriction on sample type - a rapid X-ray centring routine
scans the full sample mount to locate and centre crystals
(crystals <20um are regularly seen)
If there is a crystal there MASSIF will find it!
4. Data collections can be tailored to each sample (resolution,
space group, beam size, redundancy, SAD and more) via the ISPyB
diffraction plan (http://www.esrf.fr/MXPressE)
5. Full results available in real time via ISPyB
6. No need to come to the beamline
7. Time deducted as a function of the number of samples processed
MASSIF-1 has a beam of 100 x 65 um (HxV), that can be defined by
apertures from 50 um down to 10 um, and has a fixed energy of 12.8
keV with a flux of ~3 x 10e12 ph/s and a PILATUS3 2M detector.
The service has now been running for 2 years and is proving very
popular, we have now processed over 22,000 crystals and 20
structures have been solved de novo by SAD. Details of the process
and the beamline can be found in some recent publications:
Svensson, O., Monaco, S., Popov, A. N., Nurizzo, D. & Bowler, M. W. (2015). The fully automatic characterization and data collection from crystals of biological macromolecules, Acta Cryst. D71, 1757-1767, http://dx.doi.org/10.1107/S1399004715011918 Bowler, M.W., Nurizzo, D., et al. (2015). MASSIF-1: A beamline dedicated to the fully automatic characterisation and data collection from crystals of biological macromolecules, J. Synchrotron Rad. 22, 1540-1547, http://dx.doi.org/10.1107/S1600577515016604. Bowler, M.W., Svensson, O. & Nurizzo, D. (2016): Fully automatic macromolecular crystallography: the impact of MASSIF-1 on the optimum acquisition and quality of data, Crystallography Reviews, http://dx.doi.org/10.1080/0889311X.2016.1155050. Nurizzo, D., Bowler M.W., et al. (2016) RoboDiff: combining a sample changer and goniometer for highly automated macromolecular crystallography experiments Acta Cryst D72, 966-975, http://dx.doi.org/10.1107/S205979831601158X
For any further questions please don't hesitate to contact me or
Didier Nurizzo ([log in to unmask]). Best wishes,
Matthew.
Web:http://www.esrf.eu/MASSIF1
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FAQ:http://www.esrf.eu/MASSIF_FAQ
-- Matthew Bowler Synchrotron Crystallography Group European Molecular Biology Laboratory 71 avenue des Martyrs CS 90181 F-38042 Grenoble France =================================================== Tel: +33 (0) 4.76.20.76.37 Fax: +33 (0) 4.76.20.71.99 http://www.embl.fr/ http://www.esrf.eu/MASSIF1 https://twitter.com/id30_massif1 http://www.embl.fr/research/unit/mccarthy/members/index.php?s_personId=CP-60015559 ===================================================