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Hi, If you prefer to continue with phaser and the poly Ala model, you can obtain the poly Ala from the original pdb using chainsaw in ccp4. Also if C2 seems to be identified by xtriage and pointless, and you have not enough "space" in the unit cell for the entire molecule, maybe your protein was subject to proteolysis. In such situations, you can try with a partial model (remove loop, N or C ter etc...) I encountered this situation with coil-coil protein. In my opinion 68% of solvent content is not a low solvent content. And we can imagine two partially proteolized molecules in the ASU with a lower solvent content (because of the pretty good resolution (2A). Hope this help. And as suggest by different ccp4bb members, you should try Arcimboldo, that's the perfect case for this program. Nicolas On 20/09/2016 08:09, Abhishek Jalan wrote: > Hi > > Both xtriage and pointless suggest a C2 space group. When I tried phaser with the same data indexed in C2 space group, it yields two solutions (LLG=672, TFZ=7.6) both containing a single triple helix in the asymmetric unit. This corresponds to a rather low solvent content of 68% for such a resolution. Refinement statistics for the top solution in C2 are not encouraging either. > > start final > --------------------------------------- > R-work: 0.5522 0.5020 > R-free: 0.5162 0.5403 > RMS(angles): 1.82 1.80 > RMS(bonds): 0.013 0.011 > > Where can I get a polyA model to try in phaser. Can I build one in pymol and use it? > > Unfortunately, there is no anomalous signal.