 |
 |
Hi All, Thank you very much for the suggestions! On Tue, Sep 27, 2016 at 4:37 PM, abhimanyu singh <[log in to unmask]> wrote: > Hi Alex, > > You may also think of gradually increasing PEG3350 concentration to around > 40% which has worked pretty well in some of my cases. > > Greetings, > Abhi > > > > Abhimanyu Kumar Singh, Ph.D. > School of Biosciences > University of Kent > Canterbury, UK. > > On 27 Sep 2016 23:43, "Alex Lee" <[log in to unmask]> wrote: > >> Hi CCP4bb members, >> >> I have a condition for my protein crystals at "0.2M sodium thiocyanate, >> 25% PEG3350, pH6.9". >> >> I'd like to try cryoprotectant with just higher concentration of sodium >> thiocyanate, but I have no google hits of the range of concentration I >> should use. >> >> I wonder anyone in this forum tried to use sodium thiocyanate as >> cryoprotectant? What's the concentration range for this if used as >> cryoprotectant? >> >> Thanks. >> >