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Dear Expert, I have run fix on ICA.feat output in single subject data. I used standard.RData as training files. It has created fix folder in which I found the files given below. /home/fsl/Desktop/VMWare_share/nm/nm11_mcf_feat_ICA.feat/fix/edge1.nii.gz /home/fsl/Desktop/VMWare_share/nm/nm11_mcf_feat_ICA.feat/fix/edge2.nii.gz /home/fsl/Desktop/VMWare_share/nm/nm11_mcf_feat_ICA.feat/fix/edge3.nii.gz /home/fsl/Desktop/VMWare_share/nm/nm11_mcf_feat_ICA.feat/fix/edge4.nii.gz /home/fsl/Desktop/VMWare_share/nm/nm11_mcf_feat_ICA.feat/fix/edge5.nii.gz /home/fsl/Desktop/VMWare_share/nm/nm11_mcf_feat_ICA.feat/fix/fastsg_mixeltype.nii.gz /home/fsl/Desktop/VMWare_share/nm/nm11_mcf_feat_ICA.feat/fix/fastsg_pve_0.nii.gz /home/fsl/Desktop/VMWare_share/nm/nm11_mcf_feat_ICA.feat/fix/fastsg_pve_1.nii.gz /home/fsl/Desktop/VMWare_share/nm/nm11_mcf_feat_ICA.feat/fix/fastsg_pve_2.nii.gz /home/fsl/Desktop/VMWare_share/nm/nm11_mcf_feat_ICA.feat/fix/fastsg_pveseg.nii.gz /home/fsl/Desktop/VMWare_share/nm/nm11_mcf_feat_ICA.feat/fix/fastsg_seg.nii.gz /home/fsl/Desktop/VMWare_share/nm/nm11_mcf_feat_ICA.feat/fix/features.csv /home/fsl/Desktop/VMWare_share/nm/nm11_mcf_feat_ICA.feat/fix/features.mat /home/fsl/Desktop/VMWare_share/nm/nm11_mcf_feat_ICA.feat/fix/features_info.csv /home/fsl/Desktop/VMWare_share/nm/nm11_mcf_feat_ICA.feat/fix/highres2std.mat /home/fsl/Desktop/VMWare_share/nm/nm11_mcf_feat_ICA.feat/fix/hr2exf.nii.gz /home/fsl/Desktop/VMWare_share/nm/nm11_mcf_feat_ICA.feat/fix/hr2exfTMP.nii.gz /home/fsl/Desktop/VMWare_share/nm/nm11_mcf_feat_ICA.feat/fix/hr2exfTMP.txt /home/fsl/Desktop/VMWare_share/nm/nm11_mcf_feat_ICA.feat/fix/logMatlab.txt /home/fsl/Desktop/VMWare_share/nm/nm11_mcf_feat_ICA.feat/fix/maske1.nii.gz /home/fsl/Desktop/VMWare_share/nm/nm11_mcf_feat_ICA.feat/fix/maske2.nii.gz /home/fsl/Desktop/VMWare_share/nm/nm11_mcf_feat_ICA.feat/fix/maske3.nii.gz /home/fsl/Desktop/VMWare_share/nm/nm11_mcf_feat_ICA.feat/fix/maske4.nii.gz /home/fsl/Desktop/VMWare_share/nm/nm11_mcf_feat_ICA.feat/fix/maske5.nii.gz /home/fsl/Desktop/VMWare_share/nm/nm11_mcf_feat_ICA.feat/fix/std1mm2exfunc0.nii.gz /home/fsl/Desktop/VMWare_share/nm/nm11_mcf_feat_ICA.feat/fix/std1mm2exfunc0dil.nii.gz /home/fsl/Desktop/VMWare_share/nm/nm11_mcf_feat_ICA.feat/fix/std1mm2exfunc0dil2.nii.gz /home/fsl/Desktop/VMWare_share/nm/nm11_mcf_feat_ICA.feat/fix/std1mm2exfunc1.nii.gz /home/fsl/Desktop/VMWare_share/nm/nm11_mcf_feat_ICA.feat/fix/std1mm2exfunc1dil.nii.gz /home/fsl/Desktop/VMWare_share/nm/nm11_mcf_feat_ICA.feat/fix/std1mm2exfunc1dil2.nii.gz /home/fsl/Desktop/VMWare_share/nm/nm11_mcf_feat_ICA.feat/fix/std1mm2exfunc2.nii.gz /home/fsl/Desktop/VMWare_share/nm/nm11_mcf_feat_ICA.feat/fix/std1mm2exfunc2dil.nii.gz /home/fsl/Desktop/VMWare_share/nm/nm11_mcf_feat_ICA.feat/fix/std1mm2exfunc2dil2.nii.gz /home/fsl/Desktop/VMWare_share/nm/nm11_mcf_feat_ICA.feat/fix/std1mm2exfunc3.nii.gz /home/fsl/Desktop/VMWare_share/nm/nm11_mcf_feat_ICA.feat/fix/std1mm2exfunc3dil.nii.gz /home/fsl/Desktop/VMWare_share/nm/nm11_mcf_feat_ICA.feat/fix/std1mm2exfunc3dil2.nii.gz /home/fsl/Desktop/VMWare_share/nm/nm11_mcf_feat_ICA.feat/fix/std2exfunc.mat /home/fsl/Desktop/VMWare_share/nm/nm11_mcf_feat_ICA.feat/fix/std2highres.mat However I found an error report in the terminal "No valid labelling file specified". [fsl@fslvm6 VMWare_share]$ /usr/local/fix1.06/fix nm/nm11_mcf_feat_ICA.feat/ /usr/local/fix1.06/training_files/Standard.RData 20 FIX Feature extraction for Melodic output directory: nm/nm11_mcf_feat_ICA.feat/ create edge masks run FAST registration of standard space masks extract features FIX Classifying components in Melodic directory: nm/nm11_mcf_feat_ICA.feat/ using training file: /usr/local/fix1.06/training_files/Standard.RData and threshold 20 No valid labelling file specified [fsl@fslvm6 VMWare_share]$ Since I am using FIX for the first time I am finding difficult to proceed further with large number of dataset. Could you please help me to remove noise/artefacts from data and proceed further. Thanks in advance Ramesh