Lunds Protein Production Platform (LP3, www.lu.se/lp3) is a centre of Lund University that hosts equipment and expertise for the production, purification, characterization and crystallisation of proteins. LP3 is a service unit offering high quality protein production and crystallization for use in biological, medical and technological research. LP3 is located in the biology house in the northern campus area and close to the rich research environment offered by the faculties of science, medicine and technology. It is also close to the world unique research infrastructures MAX IV and ESS.

Job description
You will have an important role in LP3, with special responsibility for protein crystallization and characterization. This includes operation and maintenance of equipment, project planning, contact with clients and users as well as administration. You will work experimentally on a project basis; communicate continually with clients and users, research collaborators as well as your LP3 colleagues. An ability to work independently and to drive projects forward is expected. Laboratory tasks include:

• Analysis and quality control of protein samples before crystallisation trials
• Production and crystallization of proteins as well as the study of their basic physical, chemical and functional properties
• To perform basic protein analysis techniques (for example SDS-PAGE, UV/Vis spectrometry, masspectrometry, immuno-blotting)
• Protein purification in several steps of tagged and non-tagged recombinant proteins for structure and/or functional studies
• Operation and maintenance of equipment for crystallisation
• Analysis, documentation and presentation/reporting of experimental data
• Hands-on supervision of visiting guest researchers in the laboratory
• Continuous communication and dialogue with clients and users
• To keep up a state of the art knowledge and current literature knowledge to adapt and/or develop new structural methods and experiment designs

Specific tasks will include:

• Operation and maintenance of Rigaku Minstrel HTUV and Gallery 700.
• Operation and maintenance of Freedom EVO pipetting station.
• Operation and maintenance of TTP Labtech Mosquito LCP pipetting station.

This is a permanent position with a trail period of 6 months

Qualifications
The successful candidate is a skilled, organized, service-minded life-science professional who enjoys laboratory-based projects in a high-paced, team-work environment with tight deadlines. 

Minimal requirements:

• Ph.D. in biomedicine, biochemistry, molecular biology, biophysics or other relevant subject
• Five years or more experience of protein crystallisation and characterisation
• Experience in laboratory automation and robotics
• A strong interest to learn and develop new techniques in protein crystallisation and characterisation
• Experience with computers, software and databases for protein crystallisation.
• Ability to carry out all laboratory operations in a safe and effective manner and to maintain a clean and orderly laboratory environment.
• Excellent communication skills (both written and oral)
• Experience in presenting trade, results and methods
• Fluent in english, both written and oral
• Ability to be an effective team player
• Customer and service orientated
• Experience how to contribute to the learning of others

Desired but not required:

• Experience of structure determination
• Experience of advanced techniques for biophysical and biochemical characterisation (for example MS, UV-CD, SAXS/SANS, SEC-LLS, SPR, calorimetry).
• Experience with the crystallisation of membrane proteins
• Handling of eukaryotic expression systems for example Pichia pastoris, Saccharomyces cerevisiae, mammalian and insect cell lines
• Protein purification in several steps of tagged and non-tagged recombinant protein for structure and/or functional studies
• Experience with documentation using electronic lab-books
• Project planning and execution
• Budget planning and invoicing

For more information about LP3
http://biology.lu.se/services/lp3-lund-protein-production-platform