 |
 |
hi Eleanor, i have tried in P21, trying now in P2, the beta angle is 100. have also tried phenix.hyss but again only in P21. thank you for your help Alice ________________________________ From: Eleanor Dodson [[log in to unmask]] Sent: 16 August 2016 14:46 To: Douangamath, Alice (DLSLtd,RAL,LSCI) Cc: ccp4bb Subject: Re: [ccp4bb] experimental phasing with a pseudo-translation In P2 and P21? Eleanor Do you have the beta angle for your P21 cell? Eleanor On 16 August 2016 at 14:25, <[log in to unmask]<mailto:[log in to unmask]>> wrote: Yes we have tried SHELXD From: Eleanor Dodson [mailto:[log in to unmask]<mailto:[log in to unmask]>] Sent: 16 August 2016 14:21 To: Douangamath, Alice (DLSLtd,RAL,LSCI) Cc: ccp4bb Subject: Re: [ccp4bb] experimental phasing with a pseudo-translation Hmm - pseudo translation shouldnt matter for finding ha sites.. of course your SG could be wrong - a translation of 0.33 0.5 0.72 means you will have absences along 0 k 0 whether the SG is P2 or P21 I presume you have tried SHELXD? Eleanor On 16 August 2016 at 13:43, Alice Douangamath <[log in to unmask]<mailto:[log in to unmask]>> wrote: Hi all, We are trying to phase a structure using experimental phasing (few different heavy atoms showing anomalous signal to 4-5A at best (otherwise 6-7A)) and native data are about 2.8-3.0A resolution. There is no homologous model for molecular replacement and Xtriage clearly showed the presence of a pseudo-translation. The crystallographic info is: Space group P21, cell parameters: 77,134,84,90,100,90 Pseudo-translation: 0.33 0.5 0.72 So far, we did not manage to find the sites. How are the current softwares dealing with pseudo-translation for experimental phasing? Can you point us to some structures solved by experimental phasing with a pseudo-translation? Especially how it was solved? Thank you Kamel and Alice -- This e-mail and any attachments may contain confidential, copyright and or privileged material, and are for the use of the intended addressee only. If you are not the intended addressee or an authorised recipient of the addressee please notify us of receipt by returning the e-mail and do not use, copy, retain, distribute or disclose the information in or attached to the e-mail. Any opinions expressed within this e-mail are those of the individual and not necessarily of Diamond Light Source Ltd. Diamond Light Source Ltd. cannot guarantee that this e-mail or any attachments are free from viruses and we cannot accept liability for any damage which you may sustain as a result of software viruses which may be transmitted in or with the message. Diamond Light Source Limited (company no. 4375679). Registered in England and Wales with its registered office at Diamond House, Harwell Science and Innovation Campus, Didcot, Oxfordshire, OX11 0DE, United Kingdom -- This e-mail and any attachments may contain confidential, copyright and or privileged material, and are for the use of the intended addressee only. If you are not the intended addressee or an authorised recipient of the addressee please notify us of receipt by returning the e-mail and do not use, copy, retain, distribute or disclose the information in or attached to the e-mail. Any opinions expressed within this e-mail are those of the individual and not necessarily of Diamond Light Source Ltd. Diamond Light Source Ltd. cannot guarantee that this e-mail or any attachments are free from viruses and we cannot accept liability for any damage which you may sustain as a result of software viruses which may be transmitted in or with the message. Diamond Light Source Limited (company no. 4375679). Registered in England and Wales with its registered office at Diamond House, Harwell Science and Innovation Campus, Didcot, Oxfordshire, OX11 0DE, United Kingdom