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Try freezing using oil as a cryoprotectant.

Alexandra


On 8/3/16 9:20 PM, 张士军 wrote:
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Hi all

   I have a crystal grown in 35%isopropanol ,5%PEG1000/8000，pH5.5 .Because isopropanol is very easy to volatilize ,so it hard to harvest ,and it's easy to damage the crystals. Can anyone give me some suggestions about how to harvest this kind of crystals,or the detail process and buffer to harvest them .Thanks a lot !!!!!

                                                                                                                                       ShijunZhang

                                                                                                                       School of Life Science

                                                                                                                            Xiamen University


-- 
Alexandra M. Deaconescu, B.E., Ph.D.
Assistant Professor of Molecular Biology, Cell Biology and Biochemistry