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Hi Matthew, Thanks for your reply. The parcels combo is a labeled image with uniquely labeled parcels covering the whole brain (grey matter).. I've included below the output from running "fslhd Parcels_combo.nii", in case that helps with more information about it. The one "Parcels_combo" image is intended to be used across all subjects, so that time series from the same regions are extracted across all subjects. Best wishes, Claire filename Parcels_combo.nii sizeof_hdr 348 data_type FLOAT64 dim0 4 dim1 91 dim2 109 dim3 91 dim4 1 dim5 1 dim6 1 dim7 1 vox_units mm time_units ms datatype 64 nbyper 8 bitpix 64 pixdim0 0.000000 pixdim1 2.000000 pixdim2 2.000000 pixdim3 2.000000 pixdim4 1000.000000 pixdim5 0.000000 pixdim6 0.000000 pixdim7 0.000000 vox_offset 352 cal_max 375.0000 cal_min 0.0000 scl_slope 0.000000 scl_inter 0.000000 phase_dim 0 freq_dim 0 slice_dim 2 slice_name Unknown slice_code 0 slice_start 0 slice_end 0 slice_duration 0.000000 time_offset 0.000000 intent Unknown intent_code 0 intent_name huh? intent_p1 0.000000 intent_p2 0.000000 intent_p3 0.000000 qform_name Scanner Anat qform_code 1 qto_xyz:1 2.000000 0.000000 0.000000 -90.000000 qto_xyz:2 0.000000 2.000000 0.000000 -126.000000 qto_xyz:3 0.000000 0.000000 2.000000 -72.000000 qto_xyz:4 0.000000 0.000000 0.000000 1.000000 qform_xorient Left-to-Right qform_yorient Posterior-to-Anterior qform_zorient Inferior-to-Superior sform_name Scanner Anat sform_code 1 sto_xyz:1 2.000000 0.000000 0.000000 -90.000000 sto_xyz:2 0.000000 2.000000 0.000000 -126.000000 sto_xyz:3 0.000000 0.000000 2.000000 -72.000000 sto_xyz:4 0.000000 0.000000 0.000000 1.000000 sform_xorient Left-to-Right sform_yorient Posterior-to-Anterior sform_zorient Inferior-to-Superior file_type NIFTI-1+ file_code 1 descrip FreeSurfer matlab