I would definitively try a shot at room temp. The smearing might be a property of your crystals, not of the freezing.

If the room temperature pictures look good, you could consider collecting a room temp data set using multiple crystals or work on your freezing conditions.

If the room temperature pictures are smeared as well, you have to work on the crystallization conditions (additives etc.) or try to process the smeared data set (if the smearing is not too bad).

Good luck!

Herman

Von: CCP4 bulletin board [mailto:[log in to unmask]] Im Auftrag von ???
Gesendet: Montag, 25. Juli 2016 04:35
An: [log in to unmask]
Betreff: Re: [ccp4bb] cyrobuffer

yes ,i had try both small and big crystals ,and they were same situation ,and i freeze in the LN2.does that mean my crystals are not suit in freeze circumstance ,can i try RM diffraction ?

在2016-07-25 08:30:40，张士军[log in to unmask]写道：

How big are your crystals? Try small er ones if you used huge ones.

50%MPD freezes clear in my hands.

Do you freeze in the stream or LN2?

Jürgen 

......................
Jürgen Bosch
Johns Hopkins University
Bloomberg School of Public Health
Department of Biochemistry & Molecular Biology
Johns Hopkins Malaria Research Institute
615 North Wolfe Street, W8708
Baltimore, MD 21205
Office: +1-410-614-4742
Lab:      +1-410-614-4894
Fax:      +1-410-955-2926
http://lupo.jhsph.edu

On Jul 24, 2016, at 8:11 PM, 张士军 <[log in to unmask]> wrote:

Hi all

   I have a crystal grown in 50%MPD 0.2M sodium citrate pH7.2 ,because MPD can be a cyrobuffer ,so  I harvested them directly .But the spots are smearing when I difftacted it .then I add 5%glycerol in the cyrobuffer when harvested it ,the result is the same .Can anyoone give me some suggestion about this situation .Thanks a lot !!!!!

                                                                                                                                       ShijunZhang

                                                                                                                       School of Life Science

                                                                                                                            Xiamen University