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yes ,i had try both small and big crystals ,and they were same situation ,and i freeze in the LN2.does that mean my crystals are not suit in freeze circumstance ,can i try RM diffraction ?

在2016-07-25 08:30:40，张士军[log in to unmask]写道：

How big are your crystals? Try small er ones if you used huge ones.
50%MPD freezes clear in my hands.
Do you freeze in the stream or LN2?
Jürgen 
......................
Jürgen Bosch
Johns Hopkins University
Bloomberg School of Public Health
Department of Biochemistry & Molecular Biology
Johns Hopkins Malaria Research Institute
615 North Wolfe Street, W8708
Baltimore, MD 21205
Office: +1-410-614-4742
Lab:      +1-410-614-4894
Fax:      +1-410-955-2926
http://lupo.jhsph.edu

On Jul 24, 2016, at 8:11 PM, 张士军 <[log in to unmask]> wrote:

Hi all

   I have a crystal grown in 50%MPD 0.2M sodium citrate pH7.2 ,because MPD can be a cyrobuffer ,so  I harvested them directly .But the spots are smearing when I difftacted it .then I add 5%glycerol in the cyrobuffer when harvested it ,the result is the same .Can anyoone give me some suggestion about this situation .Thanks a lot !!!!!
                                                                                                                                       ShijunZhang
                                                                                                                       School of Life Science
                                                                                                                            Xiamen University