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It is potentially possible, but the low resolution tends to present challenges and I don’t have much experience with animal ASL registration. You will probably find that the more you can get a good initial alignment, e.g. manually getting the low and high res images aligned, the better any automated registration (restricted to a narrow search range) should work. Michael > On 22 Jun 2016, at 19:49, SUBSCRIBE FSL Anonymous <[log in to unmask]> wrote: > > Dear FSL experts, > > We have few animal ASL data. According to guideline, we have got ASL-perfusion map, however, its resolution was very low. > > I am wondering if I can registration ASl-perfusion map into higher resolution structural images? > > Thanks > Shiyao