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The FIRST FSL course material online indicates that volume would be calculated from a mask/ROI of the total output_name_all_fast_origsegs.nii.gz or on individual structures (e.g L_Hipp_corr.nii.gz). To assess a group of subcortical structures (e.g. L_Accu L_Amyg L_Caud L_Hipp) between groups it looks like the "for loop" could be used, as indicated in the FIRST user guide, and masks made of each individual's all_fast_origsegs.nii.gz output file; the mask volumes could then be calculated and compared between or among groups in Matlab or some other program. Also, while the structure labels and locations distinguish the individual structures, the all_fast_origsegs.nii.gz output file is a collective of all the structures; masks of individual structures can not be readily made. Further, to compare individual subcortical structures between (or among) groups, masks of individual structures can be made from running first on individual subjects and individual structures; again all volume could be grouped and calculated in Matlab or some other program. Are these assumptions correct? Lance