Dear all,

thanks to all for the info.
Off-list it was also pointed out that phenix automatically saves the pdb file to hybrid36.
I used the phenix pdb.tools to save and renumber the combined (>100 000 atoms file) and coot seems to be very happy with the results.

Cheers
Marcus

Marcus Fislage, PhD

Howard Hughes Medical Institute (HHMI)
Columbia University
Department of Biochemistry and Biophysics
Lab of Joachim Frank
New York, NY

email address: [log in to unmask]
Phone: 212.305.9524
Fax: 212.305.9500

From: Bruno KLAHOLZ [[log in to unmask]]
Sent: Tuesday, May 03, 2016 1:25 PM
To: Fislage, Marcus; [log in to unmask]
Subject: RE: Using coot with more than 100 000 atoms

Dear Marcus,

you have to rename the chain IDs for the small and large subunits to include S and L (see for example pdb entry 4UG0) to have unique names for each protein.

Best,

Bruno

###########################################################################

Bruno P. Klaholz

Centre for Integrative Biology

Department of Integrated Structural Biology
Institute of Genetics and of Molecular and Cellular Biology
IGBMC - UMR 7104 - U 964

1, rue Laurent Fries
BP 10142
67404 ILLKIRCH CEDEX
FRANCE

Tel. from abroad: 0033.369.48.52.78
Tel. inside France: 03.69.48.52.78
websites:
http://igbmc.fr/Klaholz

http://www.igbmc.fr/grandesstructures/cbi/

http://frisbi.eu

From: CCP4 bulletin board [mailto:[log in to unmask]] On Behalf Of Fislage, Marcus
Sent: Tuesday, May 03, 2016 3:06 PM
To: [log in to unmask]
Subject: [ccp4bb] Using coot with more than 100 000 atoms

Dear all,

I am working on a ribosome structure coot cuts my structure after 100 000 atoms. I heard the rumor that there is a way for coot to open a pdb file which contains more than 100 000 atoms.
However, I was not able to find anything so I would be happy if anyone could give me a pointer.

Cheers
Marcus