 |
 |
Hello,
Personally I use a combination of the CC1/2 indicator and plotting the R-meas as a function of resolution (attached plot) on which
I try to locate an inflexion point. Usually when you only integrate noise (no Bragg spots whatsoever) then this plot shoots up in the vertical direction. This isn’t really the case here (so that you may have had “proper” Bragg diffraction spots to the highest
resolution allowed by your detector and geometry of data collection), so as was suggested before I would attempt to try several resolution ranges and see how it fares. Resolution cutoff is “soft” except in a very few cases where you have absolutely no diffraction
beyond a given resolution.
HTH,
Fred.
From: CCP4 bulletin board [mailto:[log in to unmask]]
On Behalf Of Peng
Sent: Tuesday, May 3, 2016 8:23 AM
To: [log in to unmask]
Subject: [ccp4bb] How to decide the data cutoffs using CC1/2
Dear all
I am confused about how to decide the data cutoffs using CC1/2.
What resolution the following data should be used?
Shell Lower Upper Average Average Norm. Linear Square
limit Angstrom I error stat. Chi**2 R-fac R-fac Rmeas Rpim CC1/2 CC*
2.58 2.50 180.2 18.0 17.3 2.505 0.246 0.250 0.297 0.163 0.916 0.978
2.50 2.43 145.0 17.5 17.1 2.244 0.290 0.282 0.348 0.190 0.911 0.976
2.43 2.37 123.8 17.8 17.5 2.171 0.342 0.343 0.411 0.225 0.871 0.965
2.37 2.31 100.0 17.7 17.4 1.960 0.402 0.388 0.482 0.262 0.854 0.960
2.31 2.26 90.3 18.2 18.0 1.903 0.448 0.420 0.537 0.292 0.827 0.951
2.26 2.21 80.7 18.8 18.6 1.868 0.500 0.516 0.601 0.327 0.760 0.929
2.21 2.16 62.7 19.1 19.0 1.912 0.687 0.762 0.823 0.448 0.652 0.889
2.16 2.12 50.4 19.6 19.5 1.808 0.856 0.924 1.030 0.564 0.486 0.809
2.12 2.09 40.5 19.2 19.1 1.577 0.981 1.062 1.177 0.639 0.429 0.775
2.09 2.05 36.9 19.8 19.8 1.560 1.064 1.182 1.277 0.695 0.355 0.724
All reflections 991.1 35.1 26.9 2.730 0.085 0.062 0.103 0.057
Many thanks,
Peng
Upozornění: Není-li v této zprávě výslovně uvedeno jinak, má tato E-mailová zpráva nebo její přílohy pouze informativní charakter. Tato zpráva ani její přílohy v žádném ohledu Biotechnologický ústav AV ČR, v. v. i. k ničemu nezavazují. Text této zprávy nebo
jejích příloh není návrhem na uzavření smlouvy, ani přijetím případného návrhu na uzavření smlouvy, ani jiným právním jednáním směřujícím k uzavření jakékoliv smlouvy a nezakládá předsmluvní odpovědnost Biotechnologického ústavu AV ČR, v. v. i.
Disclaimer: If not expressly stated otherwise, this e-mail message (including any attached files) is intended purely for informational purposes and does not represent a binding agreement on the part of Institute of Biotechnology CAS. The text of this message and its attachments cannot be considered as a proposal to conclude a contract, nor the acceptance of a proposal to conclude a contract, nor any other legal act leading to concluding any contract; nor does it create any pre-contractual liability on the part of Institute of Biotechnology CAS