 |
 |
Hi Rado, You could fake an old run by putting 2 unfil.mrc maps (one for each half) in the output directory with the same name as you would get from the shiny half-recconstructions. Also, you would have to rename your particles as they would be called in the shiny.star file. Things would be easier from the movie-refinement, but see the betagal tutorial data for examples of how files are called if you want to avoid that. Then you could continue the old run (i.e. do NOT provide --dont_read_old_files), and use the beamtilt refinement options. There is only 1 beamtilt for the entire data set. If you think you have different tilts for different subsets of the data: divide that shiny.star file in multiple subsets and run the program on them separately. The only thing you have to see whether beamtilt improves the reconstruction is the gold-standard FSC. Optimising on it is a little bit dangerous, but only 2 parameters for a data set shouldn't hurt. HTH, Sjors On 04/27/2016 09:30 AM, Radostin Danev wrote: > Hi Sjors and everyone, > > The Relion particle polisher has command-line options for beam tilt and > defocus refinement and I was wondering if it is possible to try those > without the movie processing part? > > Thanks and best regards, > > Rado -- Sjors Scheres MRC Laboratory of Molecular Biology Francis Crick Avenue, Cambridge Biomedical Campus Cambridge CB2 0QH, U.K. tel: +44 (0)1223 267061 http://www2.mrc-lmb.cam.ac.uk/groups/scheres